madin darby bovine kidney mdbk cells  (ATCC)

Journal: Frontiers in Immunology

Article Title: A trans-activating transduction peptide fused nanobody targeting viral nonstructural protein NS4B inhibits bovine viral diarrhea virus replication

doi: 10.3389/fimmu.2025.1712683

Figure Lengend Snippet: Intracellularly-expressed Nbs inhibits BVDV replication. Characterization of MDBK cell lines were performed by fluorescent microscopy (A) . Growth curves of MDBK-Nbs EGFP cell lines (B) . BVDV-induced CPEs were monitored under an inverted microscope (C) . BVDV replication levels were determined by using IFA (D) and the virus titers in the culture supernatants were measured based on the TCID 50 value (E) . (F) Schematic diagram of different truncated C terminal of BVDV NS4B proteins. (G) The interaction of Nb91 with different truncated C terminal of NS4B proteins was determined using western blotting. The scale bar indicates 100 μm. p -values were calculated using Student's t test. An asterisk indicates a comparison with the indicated control. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: HEK-293T and Bovine endometrial epithelial (Bend) cells were procured from the China Center for Type Culture Collection (CCTCC, Beijing), and MDBK cell lines were procured from the American Type Culture Collection (ATCC CCL-22).

Techniques: Microscopy, Inverted Microscopy, Virus, Western Blot, Comparison, Control